摘要
采用反相高效液相色谱法对烟草及土壤中氟吗啉进行残留量分析。样品采用丙酮提取,乙酸乙酯-正己烷(体积比1:1)萃取,Florisil层析柱净化,紫外检测器(UVD)测定,检测波长为242nm,流动相为甲醇-水(体积比60:40)。实验结果表明:氟吗啉的最低检出限为6.2×10-10g,在烟叶中的平均回收率为95.88%～97.06%,相对标准偏差3.05%～4.14%;在土壤中的平均回收率为93.38%～95.12%,相对标准偏差2.67%～4.13%。
The residues of flumorph in tobacco leaves and soil were analyzed by using high performance liquid chromatography. The samples were distilled by acetone, and further extracted with ethyl acetate-hexane（1：1, by vol）by using UV detector at 242 nm, the extract was cleaned-up with florisil cartridge. The liquid phase was methanol-water（60：40, by vol）. The analytical results showed that the detection limit was 6.2×10-10 g under the conditon of this method, the recovery ratios of flumorph in tobacco was 95.88-97.06% and the relative standard deviations was 3.05-4.14%; the recovery ratios of flumorph in soil was 93.38-95.12% and the relative standard deviations was 2.67-4.13%.
出处
《农药》
CAS
北大核心
2010年第4期275-276,289,共3页
Agrochemicals
关键词
烟草
土壤
高效液相色谱
氟吗啉
残留分析
tobacco
soil
high performance liquid chromatography
flumorph
residue analysis
