摘要
目的：ＢＨＲＦ１的研究对于认识ＥＢＶ有关肿瘤和其它疾病的发病机理、寻找预防、治疗途径有十分重要的意义。方法：以Ｂ９５８细胞ＤＮＡ为模板，设计一对引物，用ＰＣＲ方法扩增ＢＨＲＦ１基因，用目的基因内的两个限制性内切酶位点进行酶切鉴定。结果：扩增产物长５９２ｂｐ，与预期结果一致，两个酶所切下的四个片段长度也与理想值相符。结论：本研究首次采用ＰＣＲ方法，成功地获得完整的ＥＢ病毒ＢＨＲＦ１基因，从而为该基因的进一步研究和应用奠定基础。
Objective:To study the function of BHRF 1 gene for elucidating the mechanism of EBV related tumours and other diseases,their prevention and treatment.Methods:The BHRF 1 gene was amplified from template DNA of B 95 8 cell by polymerase chain reaction,and was identified with method of digestion with two unique restriction endonucleases.Results:The amplified fragment was about 592 bp in length.The base pairs of these four bands which cleaved with two unique restriction endonucleases are in coincidence with lengthes as predicted.Conclusions:The intact BHRF 1 gene,for the first time,is obtained by using PCR technique and primers designed by ourselves.The study provides a model for further research on EBV BHRF 1 gene.
出处
《蚌埠医学院学报》
CAS
1998年第5期298-299,F002,共3页
Journal of Bengbu Medical College
基金
国家教育部博士点基金
