摘要
目的比较和评价筋膜软骨细胞和骨膜、关节软骨移植修复关节软骨大面积缺损的能力和生物特性。方法用冻存和新鲜的异体筋膜上培养的软骨细胞、骨膜和关节软骨移植修复大面积关节软骨缺损，通过大体标本、光学显微镜、扫描和透射电镜、放射自显影、微量元素和柱层析氨基酸定量测定、一氧化氮含量测定等多种观察方法进行评价。结果新鲜和冻存的筋膜软骨细胞移植在结构、形成新的软骨组织能力、代谢活性方面均优于游离软骨细胞移植；新鲜的自体骨膜和软骨移植同样可获得较好的结果。结论软骨细胞可以在筋膜上正常生长。
Objective To compare and evaluate potentialities and biological characteristics of grafts of chondrocytes cultured on fascia,periosteum and articular cartilage in repairing large defects of articular cartilage. Method The large defects of articular cartilage were repaired with grafts of freezestored and fresh chondrocytes cultured on fascia,periosteum and articular cartilage,which were evaluated by a number of observation methods. Result The fresh and freezestored fascial chondrocytes were superior to free grafts of chondrocytes in potentiality of formation of new cartilaginous tissue,structure and metabolism of newly formed tissue.Relatively good results were also obtained similarly by using fresh autografts of periosteum and cartilage. Conclusion Chondrocytes can be cultured normally on fascia, which can be regarded as an ideal carrier of chondrocytes.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第8期491-494,I094,共5页
Chinese Journal of Surgery
