摘要
用分离纯化的大鼠腹腔肥大细胞（ＰＭＣ）与同源胎鼠肺成纤维细胞（ＬＦｂ）共育，对ＬＦｂ进行形态学观察和形态计量分析。结果表明：与ＰＭＣ共育的ＬＦｂ呈复层生长，单位面积内细胞计数明显增加，粗面内质网（ＲＥＲ）增生。电镜见ＰＭＣ与ＬＦｂ紧密接触和部分ＰＭＣ有脱颗粒现象。提示ＰＭＣ可促进ＬＦｂ增值，并使其功能活跃。
Mast cells(PMC)collected from peritoneal cavities of normal adult rats were co cultured with lung fibroblasts(LFb)of fetal rats.On the 5th day of co cultured,the LFb were observed by light microscope,electron microscope,and the morphometry for them was performed by computer image analysis system.The results showed:(1)The co culturing LFb growed into many layers,and the number of LFb per unit area(0 196mm 2)were 2 2 times more than that of controls( P <0 01).(2)The volume density(Vv),surface density(Sv),mean profile area(x)of rough surfaced endoplasmic reticula(RER)were larger than that of controls( P <0 01).(3)It was usually found that the PMC contact closely with LFb and some PMC showed the degranulation.It suggested that PMC might promote proliferation of LFb whose function became active.
出处
《解剖学报》
CAS
CSCD
北大核心
1998年第1期81-85,I014,共6页
Acta Anatomica Sinica
基金
国家自然科学基金
CMB基金
