摘要
为进一步研究腺苷三磷酸抑制癌细胞增殖诱导分化的作用机理，用ＡＴＰ作用于人横纹肌肉瘤单细胞克隆亚系ＲＤＬ６细胞，用免疫荧光细胞化学方法观察ＡＴＰ作用后的ＲＤＬ６细胞核内Ｐ２１蛋白表达增强，ｃ－ｍｙｃ蛋白表达降低，膜下胞质内微丝束－应力纤维组装改善，粘着斑内的主要粘附蛋白——纽蛋白（ｖｉｎｃｕｌｉｎ）表达增强，正常小鼠成肌细胞Ｃ２Ｃ１２细胞核内Ｐ２１蛋白高表达，ｃ－ｍｙｃ蛋白不表达。结果表明：ＲＤＬ６细胞Ｐ２１表达的丢失和ｃ－ｍｙｃ表达的活化与细胞的增殖和分化异常相关，从而表明Ｐ２１表达增强和ｃ－ｍｙｃ表达的降低在ＲＤＬ６细胞表型逆转过程中起重要作用。
We have reported that adenosine triphosphate (ATP) induced growth inhibition and cell differentiation in human rhabdomyosarcoma cells. To further investigate mechanisms involved in the reverse transformation of rhabdomyosarcoma cells, expression of P 21 and c myc proteins in a subclone RDL 6 originated from human rhabdomyosarcoma cell line, was studied by using immunofluorescence cytochemical methods. After treatment with ATP, increased nuclear P 21 protein immunofluorescence and reduced nuclear c myc product immunofluorescence was observed in RDL 6 cells. Increased number and organization of cytoplasmic actin stress fibers with vinculin immunofluorescence positive adhesion plaques at the termini of stress fibers were also observed. Normal myoblast C 2C 12 cells expressed intense P 21 but negative c myc while the untreated RDL 6 cells expressed weak P 21 but intense c myc in the nuclei.The above results strongly suggested that loss of P 21 and activation of c myc expression correlate with uncontrolled cell growth and aberrant differentiation in RDL 6 cells.Accordingly, it is indicated that increase of P 21 and reduction of c myc expression plays important roles in reverse transformation of phenotypes in RDL 6 cells.
出处
《解剖学报》
CAS
CSCD
北大核心
1998年第1期63-66,I011,共5页
Acta Anatomica Sinica
基金
国家自然科学基金
北京市自然科学基金
关键词
肌肉肿瘤
横纹肌肉瘤
P21基因
c-myc基因
ATP
P 21 protein
c myc product
immunofluorescence
human rhabdomyosarcoma cells
ATP
actin cytoskeleton and vinculin adhesion plaques
