摘要
粳稻品种嘉花1号种子经60Coγ射线辐照后,筛选得到一个温敏感叶色突变体MR20。该突变体的幼苗叶色在较低温度(20℃)下呈黄白色,并随着温度升高叶色由黄白逐渐转绿,其临界温度约为22.5℃,在低温(20℃)和高温(32℃)条件下叶绿素含量也存在显著差异,表明该突变体为低温导致叶绿素合成受阻的温敏感叶色突变体。遗传分析表明该突变体性状受一对隐性核基因控制,暂命名为tsc(t)。以该突变体与籼稻9311杂交的F2群体作为定位群体,利用SSR分子标记将tsc(t)基因初步定位在水稻第3染色体短臂上的分子标记RM231和RM14407之间,其遗传距离分别为1.5cM和0.5cM。随后,进一步扩大F2群体将该tsc(t)基因定位在分子标记MM0541和RM14407之间的约440kb内。
A thermo-sensitive leaf-color mutant,MR20,was discovered in progeny of a japonica variety,Jiahua No.1,induced by 60Co γ-rays irradiation.Under the conditions of low temperature（20℃ ）,the leaf-color of the mutant at seedling stage was yellow and tuned into green with the increase of temperature and its critical temperature was about 22.5℃ .In addition,the significant difference of the chlorophyll contents at two temperatures（20℃ and 32℃ ） was observed showing that MR20 was a thermo-sensitive chlorophyll-deficit mutant at the seedling stage.Genetic analysis revealed that the character of thermo-sensitive chlorophyll-deficit was controlled by a single recessive nuclear gene,tentatively designated as tsc（t）.The F2 population derived from the cross MR20 × 9311 was as materials,and SSR markers was used,the tsc（t） gene was roughly mapped at between RM231 and RM14407 molecular markers on the short arm of chromosome 3 with the genetic distance of 1.5cM and 0.5cM,respectively.With larger F2 mapping population,the tsc（t） gene was narrowed to 440kb between MM0541 and RM14407 molecular markers.
出处
《核农学报》
CAS
CSCD
北大核心
2010年第2期220-224,共5页
Journal of Nuclear Agricultural Sciences
基金
国家航天育种工程项目(2006HT10001)
上海市科委项目(08PJ14085
075405117
09DJ1400505
0939191200)
关键词
水稻
基因定位
温敏感
叶绿素缺失突变体
rice
gene mapping
thermo-sensitive
chlorophyll-deficit mutant
