摘要
应用聚丙烯酰胺凝胶垂直平板电泳对长江水系的湖北钟祥、蒲圻光亮钉螺和江陵、潜江、阳新助壳钉螺进行酯酶同工酶（EST）和苹果酸脱氢酶（MDH）电泳比较，结果显示：3地肋壳钉螺EST同工酶酶带数目、活性及Rf值完全一致；2地光亮钉螺与肋壳钉螺相比，酶谱特征也基本一致，EST同工酶仅1－3条弱带稍有差异；MDH同工酶仅蒲圻光亮钉螺与其它各地肋壳与光亮钉螺稍有差异，提示上述各地钉螺在种系进化上具有比较密切的亲缘关系。
This paper reports a comparative study on the esterase (EST) isoenzyme and malate de hydrogenase (MDH) isoenzyme of Oncomelania snails by means of slab polyacrylamide gel electrophoresis. The smooth-shell Oncomelania were collected from Zhongxiang and Puqi while the rib-shell Oncomelania were from Jiangling、 Qianjiang and Yangxin of Hubei province. All these snails were dispersed in the Yangtze River system. The results showed that the rib-shell Oncomelania from these 3 conuties were entirely identical in number of EST enzyme bands,enzyme activity and Rf values. The very similar isoenzyme patterns of EST were shown between the smooth-shell Oncomelania and rib-shell Oncomelania. A very small difference was observed in MDH isoenzyme patterns between smooth-shell Oncomelania from Puqi and rib-shell Oncomelania from the other areas. These results indicate that the Oncome lania snails from the different conuties in Hubei province have very close relationship be tween each other.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
1998年第5期267-270,共4页
Chinese Journal of Schistosomiasis Control
基金
国家自然科学基金
