摘要
细胞微环境是一个多因素组成的、时空可变的复杂集合,对细胞的行为和功能发挥起着决定性作用。但传统的细胞生物学研究方法很难在体外为细胞提供这样一个复杂的、微尺度的生长环境,致使许多体外研究结果与在体情况相差甚远。近年来,微流控技术与细胞培养技术的结合为细胞微环境的模拟和控制提供了可能。文章通过提炼微环境的重要参数及其特征,介绍微流控技术是如何满足这些参数的需求,探讨了微流控技术在体外模拟细胞微环境的可行性,并总结了近年来该技术在微环境体外模拟研究中取得的成果,对微流控技术在细胞微环境构建中的发展方向和应用前景进行了展望。
Cellular microenvironment, which is a complex integration of multiple factors, plays a key role in cells＇ behavior and function. It is rather difficult for the traditional research method of cell biology to provide a similar in-vivo environment for cell growth in vitro, which results in the inconsistencies between the facts in vivo and the research findings in vitro. With the introduction of microfluidics into cell culture technology, microfluidics system make it possible to mimic in vivo microenvironment. This paper dealt with the important factors of microenvironment and its in-vivo features, and discussed the potentiality of microfluidics to establish and control the microenvironment in vitro. Based on the findings available, the prospect of microfluidics in cellular microenvironment was also made.
出处
《生物物理学报》
CAS
CSCD
北大核心
2010年第3期209-215,共7页
Acta Biophysica Sinica
基金
国家自然科学基金面上项目(10872224
30870607)
重庆市自然科学基金项目(2008BB5192)~~
关键词
细胞微环境
微流控技术
细胞外基质
可溶性因子
机械力
Cellular microenvironment
Microfluidic technology
Extracellular matrix
Soluble factors
Mechanical force
