摘要
目的探讨Bmi-1-siRNA对乳腺癌MCF-7细胞体外增殖的作用及机制。方法将不同浓度的Bmi-1-siRNA转染MCF-7细胞,MTT法检测细胞增殖情况,免疫细胞化学技术检测转染72 h后MCF-7细胞内Bmi-1、端粒酶逆转录酶(hTERT)、细胞增殖相关核抗原Ki-67蛋白的表达。结果Bmi-1-siRNA对MCF-7细胞的生长抑制率明显升高,且随浓度增高而升高(P<0.05);MCF-7细胞中Bmi-1、hTERT、Ki-67蛋白表达明显降低(P<0.05)。结论Bmi-1-siRNA可有效抑制MCF-7细胞的增殖,其机制为降低Bmi-1 mRNA及Bmi-1、hTERT蛋白的表达。
Objective To investigate the effect and mechanism of the small interfering RNA of B-ceil specific moloney leukemia virus insert site 1 （ Bmi-1 ） on the proliferation of breast cancer MCF-7 ceils in vitro. Methods MCF-7 ceils were transfected with different concentrations of Bmi-1-siRNA-1 ,then the proliferation was detected hy MTT method, the expression of Bmi-1, human telomerase reverse transcriptase（hTERT ）and proliferation nuclear antigen Ki-67 protein were examined using immunocytochemistry method 72hs after the transfection. Results The growth inhibition rate of MCF-7 ceils increased significantly after the transfection of Bmi-1-siRNA, which showed concentration-dependant manner（P 〈 0.05 ） ;The expression of Bmi-1, hTERT and Ki-67 protein in MCF-7 cells decreased significantly （ P 〈 0.05 ）. Conclusion Bmi-1- siRNA can inhibit the proliferation of MCF-7 ceils effectively ,the mechanism maybe down-regulating the expression of Bmi- 1, hTERT protein.
出处
《山东医药》
CAS
北大核心
2010年第15期32-34,共3页
Shandong Medical Journal
