摘要
采用孕妇外周血淋巴细胞姐妹染色单体交换(SCE)技术,检测巨细胞病毒(CMV)抗体阳性孕妇淋巴细胞DNA损伤和修复.结果表明,具有不良生育史孕妇组CMV抗体阳性发生率为28.4%,未孕组为16.7%,两组间存在明显差异(P<0.05);具有不良生育史CMV抗体阴性孕妇组,外周血淋巴细胞SCE平均发生率为13.99±2.9,无不良生育史CMV抗体阴性孕妇组,SCE平均发生率为10.49±3.61,两组间存在明显差异(P<0.05).这从细胞分子水平为孕期CMV感染检测提供了一种新的方法.
We applied the sister chromatid exchange (SCE) technique to pregnant women with antibodies against cytomegalovirus (CMV) positive, to detect the lesion and repair of DNA in the lymphocytes. The results were that the pregnant group with a history of unhealthy delivery showed a CMV antibody positivity rate of 28.8%, while in the nonpregnant group the positivity rate was 16.7%, showing a significant difference between the two groups.In the pregnant group with a history of unhealthy delivery and with CMV antobodies positive, the average SCE frequency in peripheral blood was 13.99±2.9, as compared with 10.49±3.61 in the pregnant group without a history of unhealthy delivery and with CMV antibody negative. The difference was remarkable(P<0.05).This technique provides a new method for detecting CMV infection at the cellular and molecular levels and also a reliable index for the prevention and treatment of CMV infection.
出处
《青岛医学院学报》
1990年第4期263-266,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
巨细胞病毒
抗体
染色单体
妊娠
Cytomegaloviras
Antobody
Chromatid
Lymphcyte
Pregnancy
