摘要
目的观察颈内动脉输注人脐血单个核细胞(human cord blood mononuclear cells,HCMNCs)对血管性痴呆(vascular dementia,VD)大鼠的行为学改善的作用以及对VD大鼠血清脑特异性蛋白神经元特异性烯醇化酶(neuron spcific enolase,NSE)和S-100蛋白的影响。方法大鼠随机分为模型组、治疗组、对照组;各组大鼠又分为2、4、8周3个时相点。以穿梭箱对大鼠行为学进行检测。用ELISA法对大鼠血清中NSE和S-100蛋白进行检测。结果穿梭箱结果显示HCMNCs治疗组大鼠术后各时相点主动回避反应(active avoidance response,AAR)比例均显著高于模型组。治疗组大鼠术后各时相点血清NSE和S-100蛋白含量与模型组有显著性差异,与对照组比较无显著性差异。结论采用HCMNCs治疗对VD大鼠的行为学有一定的改善作用,说明HCMNCs治疗对脑组织细胞有一定的保护作用。
Objective To investigate the effect of intracarotid administration of human cord blood mononuclear cells （HCMNCs） on active avoidance response（AAR） ratio of vascular dementia rats, and the effect on neuron specific enolase （NSE） and S-100 protein of vascular dementia rats. Method The rats were randomly divided into model group, treatment group and control group. The three groups were observed at the 2nd, 4th and 8th week, respectively. Serum samples of rats were taken at 2nd, 4th and 8th after operation. NES and S-100 protein were analyzed by enzyme-linked immunoadsordent assay （ELISA）. Result Behavioral tests showed the AAR ratio in treatment group was signi^cant higher than that in model group. ELISA identified that serum NSE and S-100 protein level had not significant difference between treatment group and control group. Conclusion Behavioral tests show that HCMNCs therapy can improve cognitive function of VD rats. And intravenous administration of HCMNCs can protect neurons and glial ceils in VD rats.
出处
《实用医药杂志》
2010年第4期354-356,共3页
Practical Journal of Medicine & Pharmacy
基金
重庆市科委科研基金攻关项目[项目编号:7830(2003-2005)]
