摘要
目的：观察双氢青蒿素（ｄｉｈｙｄｒｏｑｉｎｇｈａｏｓｕ，ＤＱＨＳ）对约氏疟原虫在斯氏按蚊体内发育的影响。方法：受染疟原虫小鼠一次性经口灌喂不同浓度ＤＱＨＳ药液后，供蚊虫吸血，应用光镜和电镜观察对照组和用药组的疟原虫在蚊体内的发育情况。结果：ＤＱＨＳ对疟原虫配子体有一定的抑制作用，其作用强度与配子体成熟程度和用药剂量不同有关。未成熟配子体对药物较敏感；随着药物剂量的增加，卵囊和子孢子的阳性率和密度随之逐渐下降；但１８０ｍｇ或２４０ｍｇ／ｋｇ用药组对子孢子密度的影响的差别无显著性意义。电镜观察６０ｍｇ／ｋｇ作用１６ｈ后，用药组的蚊胃上卵囊（１２ｄ－１３ｄ解剖蚊），出现膜受损，甚至胞质空泡化。１２０ｍｇ／ｋｇ药量对蚊体内３日龄卵囊作用１６ｈ，卵囊仍继续发育，比较对照组和用药组的卵囊和子孢子密度，两组间差别无显著意义（Ｐ＞０．０５）；两组卵囊超微结构形态亦无明显差异。结论：ＤＱＨＳ影响约氏疟原虫配子体感染性而减少蚊媒传播。
AIM:To observe the effect of dihydro qinghaosu (DQHS) on the development of Plasmodium yoelii yoelii in Anopheles stephensi and to explore the possibility of whether DQHS has preventive effect against malaria.METHODS: Plasmodium yoelii yoelii infected mice that had been administered with a single dose of different dosages (60 mg/kg,120 mg/kg,180 mg/kg and 240 mg/kg) of DQHS were exposed to mosquitoes to suck blood .The development of malaria parasites in the mosquitoes of the control group and the treated group were observed by light microscopy (LM) and transmission electron microscopy (TEM). RESULTS:DQHS exhibited certain inhibitory effect on the infectivity of gametocyte. The degree of inhibition was related to the developmental stages of gametocyte and the drug dosage. Immature gametocytes were more sensitive to DQHS than the mature gametocytes. With the increases in dosage , the positive rate and the density of oocyst and sporozoite decreased.However, the difference in the density of sporozoite between 180 mg/kg and 240 mg/kg treated groups was insignificant.TEM result showed damage of the membrane system and vacuolation in the cytoplasm of the oocysts (12-13 d) on mosquito midgut of 60 mg/kg 16 h treated group. The oocysts in the mosquitoes continued to develop after treated with 120 mg/kg of DQHS for 16 h on 3 day instar. There was no significant difference ( P > 0 05) in the density of oocyst and sporozoite between the control group and the treated group.CONCLUSION:DQHS can affect the infectivity of gametocyte and decrease the malaria transmission but cannot inhibit the sorogonic stage directly.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1998年第6期421-424,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
中国中医研究院中药研究所科研基金
关键词
以氢青蒿素
药氏疟原虫
配子体感染性
斯氏按蚊
Dihydroqinghaosu, Plasmodium yoelii yoelii , infectivity of gametocyte, Anopheles stephensi , sprorogonic stage
