摘要
利用Primer 3.0设计出1对特异性引物F和R,以成年山羊基因组DNA为模板,扩增山羊的KIFI基因,将其克隆至pGEM-T载体,转化后挑取阳性菌落进行酶切及测序鉴定。结果表明,克隆所得的472 bp序列(GenBank登录号:GQ988385),包括山羊KIFI基因的Exon 1全长、部分5′UTR和部分Intron 1序列。山羊KIFI基因的Exon 1序列编码116个氨基酸,与绵羊、牛、马、人和家鼠的同源性分别为98%、90%、90%、88%和84%。KIFI基因部分序列的克隆,为进一步研究KIFI基因多态性与绵、山羊绒用性能间的相关性奠定基础。
A pair of pecific primer was designed using Primer 3.0 software to amplify the sequence of KIFI gene from goat genomic DNA.The KIFI was cloned into pGEM-T vector,positive colonies were sequenced and digested by restriction endonuclease after transforming.The results showed that the sequence length was 472 bp（No.GQ988385）,including Exon 1,part of 5′UTR and part of Intron 1 sequence of goat KIFI Gene,Exon 1 sequence coding for polypeptide contains 116 amino acid.Which shared 98%,90%,90%,88% and 84% homology with the sequences of sheep,cattle,horse,human and house mouse.Therefore,cloning of goat KIFI gene could be a basis for the further study on polymorphism and correlation on cashmere trait.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第4期91-94,共4页
China Animal Husbandry & Veterinary Medicine
基金
贵州省优秀科技教育人才省长专项资金项目(黔省专合字(2009)129)
自然基金重大研究计划(NSF9020)
毕节学院科学研究基金项目(院科合字20092022号)
