摘要
目的研究大鼠颅脑损伤后心肌损害和心肌线粒体锰超氧化物歧化酶(Mn-SOD)活性的变化及银杏叶提取物(GBE)对其影响。方法采用液压冲击建立颅脑损伤模型和药物干预模型,24 h动态监测心电图变化,测定血清肌酸磷酸激酶同工酶(CK-MB)的含量和心肌线粒体Mn-SOD的活性改变,并观察心肌HE染色的病理形态学改变。结果大鼠颅脑损伤后心电图异常发生率显著增高,血清CK-MB含量升高(P<0.05),心肌细胞线粒体Mn-SOD活性降低(P<0.05),且心肌细胞线粒体Mn-SOD活性与血清CK-MB含量呈负相关(r=-0.997,P<0.05)。GBE预处理后可以使大鼠颅脑损伤后心电图异常发生率明显降低(P<0.01),血清CK-MB含量降低(P<0.05),心肌细胞线粒体Mn-SOD活性升高(P<0.05),心肌的病理损伤程度减轻。结论大鼠颅脑损伤可引起明显的心肌损害,颅脑损伤后心肌损害的发生可能与心肌细胞线粒体抗氧化应激水平降低有关,GBE对大鼠颅脑损伤后心肌损害有保护作用。
Objective To investigate the myocardial damage and changes of myocardial mitochondrial Mn-superoxide dismutase （Mn-SOD） activity in craniocerebral injured rats and the effect of Ginkgo biloba extract （GBE） on them. Methods Craniocerebral injured rats model was established by fluid-percussion and treated with GBE. The dynamical changes of electrocardiograph （ECG） in 24 h were monitored, the serum level of MB isoenzyme of creatine kinase （CK-MB） and the change of myocardial mitochondrial Mn-SOD activity as well as the pathologic changes of myocardium （ HE staining） were observed. Results The occurrence of ECG abnormality obviously increased in the injured rats, accompanied with increased serum CK-MB （ P 〈 0.05） and decreased myocardial Mn-SOD levels （P 〈 0.05）, and the Mn-SOD activity was negatively correlated with the level of CK-MB （r= -0. 997, P 〈0.05）. Pretreatment of GBE resulted in the decrease of ECG abnormality occurrence （P 〈0.01 ）, serum CK-MB level （P 〈0.05）, and degree of myocardial damage, as well as the increase of Mn-SOD activity in post-craniocerebral injured rats. Conclusions Craniocerebral injury can result in distinct myocardial damage, which is possibly correlated with the lowering of anti-oxidation stress level of myocardial cellular mitochondria. GBE possesses the protective effect on myocardial damage after craniocerebral injury.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2010年第3期299-302,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30600637)
关键词
颅脑损伤
心肌损害
锰超氧化物歧化酶
银杏叶提取物
craniocerebral injury
myocardial damage
Mn-superoxide dismutase
Ginkgo biloba extract
