摘要
介绍了同时测定发酵乳中蛋白糖和氨基酸的ＨＰＬＣ方法。样品经甲醇除去蛋白，异硫氰酸苯衍生，ＲＰ－ＨＰＬＣ法测定。蛋白糖回收率为９４．２％～９８．７％，精密度以相对标准偏差表示为２．１５％～３．７４％。
An RP HPLC method for the simultaneous determination of aspartame and amino acids in fermented milk beverages were established. Samples were prepared by mixing with methanol at a ratio of 1∶1 and centrifuged at 4 000 r/min for 15 min. Ten μL supernatant was moved into a sample tube, dried and derivatized according to PICO TAG procedure developed by Waters. A Novapak C 18 column (3.9 mm×150 mm) was used instead of PICO TAG column and the gradient elution program was modified correspondingly. Column temperature was maintained at 38℃ and the components were detected at 254 nm. Linearity for aspartame at the range in 1 100 mg/L is A=333C+20 with r=0.999 6 where A is the integrated area of chromatographic peak of aspartame and C is the corresponding mass concentration. Repeatability for 8 injections was tested and the RSD of 3.2% was obtained. The recovery of aspartame for 5 samples with the method ranged from 94 2% to 98.7%。
出处
《色谱》
CAS
CSCD
北大核心
1998年第6期539-540,共2页
Chinese Journal of Chromatography
基金
浙江省分析测试基金
关键词
HPLC
蛋白糖
氨基酸
发酵乳制品
分析
reversed phase high performance liquid chromatography, PICO TAG system, aspartame, amino acids
