摘要
研究了氯化血红素作为过氧化物模拟酶催化显色体系（Ｃ６Ｈ５－ＯＨ－４－ＡＡＰ－Ｈ２Ｏ２）并与葡萄糖氧化反应偶联的动力学性质。通过控制模拟酶和各反应物的用量确定了反应成假一级反应的葡萄糖浓度范围，在此反应体系中，葡萄糖浓度可以通过测定染料的吸光度而获得。该法干扰小，回收率达９４％～９８％。检测限为２．０×１０－４ｇ／Ｌ。
In this paper it is reported to investigate kinetic characteristics of Color derveloping system catalyzed by haem chloride as simulation eanzyme of peroxidase and of the coupling reaction of oxidation of glucose,to determine concentration range of glucose in a apparent first order reaction under control of the conentration of the enzyme and some reactants.In this reaction system glucose concentration was determined by absorbance of a dye,and this procedue has low interference satisfactory recovery with 94%～98% and detection limit of 2.0 ×10 -4 g/L of glucose.
出处
《分析试验室》
CAS
CSCD
1998年第6期35-37,共3页
Chinese Journal of Analysis Laboratory
