摘要
从随机噬菌体九肽表位库中筛选出抑制草鱼出血病病毒（ＧｒａｓＣａｒｐＨｅｍｏｒｈａｇｅＶｉｒｕｓ，ＧＣＨＶ８７３）感染的多肽分子。采用完整、生物素化的草鱼出血病病毒颗粒作为受体，与以融合蛋白形式在丝状噬菌体ｆＵＳＥ５的外壳蛋白Ⅲ的Ｎ端表达的随机九肽库作用。经三轮体外亲和筛选（Ｂｉｏｐａｎｎｉｎｇ）及ＥＬＩＳＡ法检测后，从肽库中筛选出１６个与病毒高亲和力结合的噬菌体克隆，其中六个阳性克隆能有效地抑制病毒在ＣＩＫ细胞中的复制，并使病毒的半数组织培养感染剂量（ＴＣＩＤ５０）下降五个数量级。表明噬菌体肽库技术完全能够应用于抗病毒研究，为研制抗病毒短肽制剂打基础。
Screening inhibitors of GCHV 873 (Grass Carp Hemorrhage Virus)to inhibit its replication in CIK cells in vitro ,the biotinylated intact virion of GCHV was used to react on a random sequenced peptide library of 9 amino acids fused on protein Ⅲ of filamentous phage fUSE5 .After 3 rounds of affinity selection,immuno screening and ELISA,16 positive clones were selected using CsCl gradient purified virus particles, 6 of them inhibited the replication of GCHV in CIK cells in vitro and decreased the TCID 50 of GCHV from 10 -10 to 10 -5 .These findings open possibility of identifying virus proteins or epitopes as targets for antiviral agents.
出处
《中国病毒学》
CSCD
1998年第4期351-357,共7页
Virologica Sinica
基金
SCBA第七届国际学术大会(加拿大
多伦多
1997)资助
关键词
噬菌体九肽库
草鱼出血病毒
抗病毒多肽
Random peptide library,Biopanning in vitro ,Grass Carp Hemorrhage Virus,Antiviral peptides
