摘要
采用多相缓冲系统，在成层胶Ｔ＝５％，Ｃ＝２６％，分离胶Ｔ＝８％，Ｃ＝５％的条件下用聚丙烯酰胺凝胶电泳对人类短串联重复序列（ＳＴＲ）ＤＮＡ片段进行分离．其中，成层胶内主要缓冲成分为２二羟乙基亚胺三羟甲基甲烷（Ｂｉｓｔｒｉｓ）、Ｈ２ＳＯ４及Ｎ、Ｎ２（羟乙基）甘氨酸（Ｂｉｃｉｎｅ）；而分离胶以Ｔｒｉｓ、Ｈ２ＳＯ４及２二羟乙基亚胺三羟甲基甲烷（Ｂｉｓｔｒｉｓ）为主，构成多相缓冲系统．ＤＮＡ片段在成层胶中被有效地压缩，在分离胶内又可完全解压缩，使其按片段大小分离；
A multiphasic buffer system was used to seperate the human short tandem repeat DNA fragments.In stacking gel the main content of buffer were bistris,sulfuric acid and bicine while tris, sulfuric acid and bistris play a major role in seperating gel.The DNA fragments,which can be stacked efficiently in stacking gel and destacked completely in seperating gel, were seperated according to their size. By this way the high resolution results can be achieved.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第6期547-550,共4页
Progress In Biochemistry and Biophysics
基金
山西省青年自然科技基金
山西省教委青年学科带头人培养对象基金
关键词
短串联重复序列
电泳
成层胶
分离胶
stacking gel, seperating gel, short tandem repeat DNA fragment
