摘要
利用扩大的籼粳杂交来源（窄叶青８号×京系１７）的水稻（ＯｒｙｚａｓａｔｉｖａＬ．）加倍单倍体（ＤＨ）群体，构建了包含４４４个位点的分子连锁图谱，覆盖水稻基因组１９６２ｃＭ（ｃｅｎｔｉＭｏｒｇｏｎ），标记间的平均图距小于５ｃＭ。此图谱包括２７６个ＲＦＬＰ标记、３４个ＲＡＰＤ标记、８９个微卫星标记、１０个ＡＦＬＰ标记、２６个端粒重复相关序列（ＴＡＳ）标记以及９个同工酶标记。该遗传图谱与其它的水稻高密度遗传图谱具有较高的可比性，并有自己的特点。
A high density genetic linkage map comprised of 444 loci was constructed from a doubled haploid population derived from a inter subspecific cross between an Oryza sative L. ssp. indica variety (“Zhaiyeqing 8”) and a japonica variety (“Jingxi 17”). The genetic map consisted of 276 RFLP markers,34 RAPD markers,89 microsatellite markers,10 AFLP markers,26 markers based on telomeric repetitive associated sequence (TAS) and 9 isozyme markers. This genetic map was highly comparable with other high density rice genetic maps and had its unique feature which meritted it suitable for sustained genetic analysis.
基金
中国水稻基因组计划和美国洛克菲勒基金
关键词
水稻
分子标记
遗传图谱
交换率
偏分离
Oryza sativa, Molecular markers, Genetic map, Recombination frequency, Skewed segregation
