摘要
“明恢６３”是我国许多重要的水稻杂交组合中的恢复系。为了对其进行深入的遗传学研究以及克隆控制重要农艺性状的基因，以细菌人工染色体（ｐＢｅｌｏＢＡＣ１１）为载体，克隆经限制性内切酶消化后部分收集的“明恢６３”基因组ＤＮＡ，将连接混合物转化细菌ＤＨ１０Ｂ，构建了细菌人工染色体（ＢＡＣ）文库。该文库共有２６０００个转化子，插入片段为９０～２４０ｋｂ，平均大小为１５０ｋｂ。经计算该文库覆盖９倍水稻基因组。该文库正在用于构建几个基因所在区段的物理图谱。
Minghui 63' is the restorer line for a number of the most important commercial rice hybrids varieties in China. To facilitate long term commitment in genetic analysis and molecular cloning of the superior genes in the genome of “Minghui 63', the authors have constructed a large insert genomic DNA library using the bacterial artificial chromosome (BAC) cloning vector (pBeloBAC11). Size fractionated Hin dⅢ digest of genomic DNA was ligated to the BAC vector, and the ligation mixture was used to transform the bacterial strain DH10B. A total of over 26 000 clones were obtained with the average insert size of about 150 kb, ranging from 90 to 240 kb. These clones thus represent 9×rice haploid genome equivalents. The library is now being used for physical mapping of several genomic regions for map based gene cloning.
关键词
水稻
明恢63
BAC文库
Bacterial artificial chromosome library, Genome analysis, Gene clone
