摘要
将玉米（ＺｅａｍａｙｓＬ．）（Ｐ９１０×Ｚ３１）愈伤组织接种于含不同浓度草丁膦及不同氨基酸的Ｎ６培养基上，测定愈伤组织重量增长及ＮＨ＋４积累水平。统计结果表明，当培养基中草丁膦浓度为４ｍｇ／Ｌ时，愈伤组织重量增长最低；浓度为８ｍｇ／Ｌ时，ＮＨ＋４积累水平达最高。综合考虑这两种因素，在培养基上选择转化体的草丁膦浓度以６ｍｇ／Ｌ为宜。在含有草丁膦的选择培养基上，Ｌ精氨酸和Ｌ谷氨酸能通过代谢调节显著降低玉米愈伤组织中的ＮＨ＋４浓度；而Ｌ脯氨酸虽明显促进玉米愈伤组织在选择培养基上的重量增长。
Maize ( Zea mays L.) embryogenic calli were cultured on N 6 medium and treated respectively with different concentrations of herbicide Basta and amino acids. The NH + 4 concentrations and weight increase of maize calli were measured. Statistical analysis revealed that callus weight increased less when cultured on N 6 medium with 4 mg/L of Basta while NH + 4 concentration reached its peak when cultured on N 6 with 8 mg/L of Basta. Therefore 6 mg/L of Basta was considered as the optional dosage for selection of transgenic calli L arginine and L glutamic acid significantly reduced the NH + 4 concentration in maize calli while L proline had little effects on NH + 4 concentration even though it enhanced callus weight enormously.
基金
国家自然科学基金
联合国环境署通过中国微生物资源中心部分资助
关键词
玉米
愈伤组织
氨基酸
除草剂
NH^+4
抗性
Maize embryogenic calli, N 6 medium, Amino acids, Herbicide, NH + 4
