摘要
目的:建立一个以最低保护力的抗体水平(15IU/ml)为临界值的风疹病毒特异性IgG抗体的检测方法。方法:用优选的抗原和具有变色功能的标本稀释液,用世界卫生组织提供的标准品标定标准曲线,以常规间接ELISA方法进行检测。结果:对检测方法的各个环节进行了比较研究,确定了检测方法的最佳工艺流程。对1005例育龄妇女血清标本进行检测,并与意大利Sorin公司的试剂检测结果进行比较,两种方法呈高度相关(Kappa值为0.85)。结论:本文建立的方法具有敏感、特异、简便、快速、可重复性好等特点,适用于育龄妇女风疹病毒抗体水平的筛查。
Objective：To establish a method for detecting rubella virus（RV）specific immunoglobulin G（IgG）antibodies using 15IU/ml of anti-RV-IgG defined as minimum immunity criteria of preventable RV infection.Methods：The measurement of anti-RV-IgG was conducted by indirect enzyme-linked immunosorbent assay（ELISA）using optimal RV-antigen and the sample dilution buffer with a color change function.Standard curve were calibrated with international standard for anti-rubella immunoglobulin substance provided by World Health Organization.Results：A technical research on each step of the method for detecting RV-IgG was performed.And the optimal technique flowchart was defined.The result of this method was compared with that of the assay using a Sorin kit by measuring anti-RV-IgG in the serum samples of 1 005 women of reproductive age,showing a high consistency（Kappa value：0.85）.Conclusion：The specific,sensitive and reliable method we established could be used for RV antibodies screening among women of reproductive age.
出处
《中国计划生育学杂志》
北大核心
2010年第4期209-212,共4页
Chinese Journal of Family Planning
基金
国家"十一五"科技支撑计划项目课题(2006BAI05A02)
关键词
风疹病毒
IGG抗体
酶联免疫分析
Rubella virus
Immunoglobulin G antibodies
Enzyme linked immunosorbent assay
