摘要
目的探讨用蚕丝与同种异体肌腱细胞联合培养植入体内，构建组织工程化肌腱的生物力学指标。方法实验分2组，一组是植入附着了肌腱细胞的蚕丝材料组，另一组是单纯植入蚕丝材料组。分别在术后的第2，4，6，8周进行随机取材，在每次取材时每组分别取20只，对材料进行生物力学测定。所得数据均采用SPSS13．0统计软件进行处理和分析。结果在第2，4，6，8周进行取材，生物力学的测定结果显示在同时间点内，细胞组的结果明显优于非细胞组（P≮0．05），细胞组自身在不同时间点的比较中，发现除第8周以外（P〉O．05），时间越长，力学的结果越优秀（P〈O．05）；而在非细胞组则只有第8周的结果与前3次测定结果的差异有统计学意义（P〈0．05）。结论本实验的结果说明蚕丝材料对肌腱细胞的黏附性好，生物力学性能优越，附着肌腱细胞后可以构成组织工程化肌腱。经更深入的实验和研究，蚕丝材料可能会在肌腱缺损的治疗方面具有良好的应用前景。
Objective To investigate the biomechanics of the tissue engineered tendons which use the embryonic tendon cells as the seed-cells and the silk as the scaffolds. Methods Two groups were set up with one as the group with tenocytes and the other as the group without tenocytes. Tissue engineered tendons were taken out at 2-week, 4-week, 6-week, 8-week post-operation, with 20 samples per-group each time. The values of biomechanics were measured and analyzed using the software SPSS 13.0. Results The biomechanieal properties of the tissue engineered tendons in the group with tenocytes were significantly better than those in the group without tenocytes （P〈0.05）. In the group with tenocytes, the vitodynamics results got better with the increase of implantation time （P〈0.05） except for the results of 8-week post-operation（P〉0.05）. But in the group without tenocytes, only the results of that from 8-week post-operation were of significant significance （P〈0.05）. Conclusion The results presented in the current study demonstrated that silk could stick tenocytes well, hold the characteristics of great resistance to draw after adhesion of tenocytes, and formed the tissue engineered tendon gradually in chickens, suggesting its potential application in the treatment of the defect of tendon.
出处
《国际生物医学工程杂志》
CAS
北大核心
2010年第1期40-43,共4页
International Journal of Biomedical Engineering
