摘要
目的研究针刺雌性大鼠身体各部位不同穴位对下丘脑促性腺激素释放激素(GnRH)相关神经元活动的影响,以比较身体各部位不同穴位对这类神经元活动的有效性,为针刺治疗生殖内分泌系统疾病提供实验依据。方法用玻璃微电极细胞外记录下丘脑内视前内侧区、弓状核及室旁核区域的GnRH相关神经元放电,以该神经元放电为指标,探察针刺身体不同部位的15个穴位对神经元的激活效应及激活强度的差异。结果针刺15个穴位前后兴奋性神经元放电变化百分率从大到小的排列顺序为:子宫、关元、带脉、三阴交、耳甲、足三里、下关、关元俞、肾俞、中脘、曲池、合谷、内关、肝俞、膻中;从针刺部位来看有明显变化的是:下腹部、后肢、下背部。针刺对抑制性神经元的影响同兴奋性神经元的结果基本一致。结论针刺能有效调节下丘脑-垂体-性腺内分泌轴的活性,但不同穴位的调节作用不同。穴位的调节作用与其所处部位的神经节段支配密切相关,与生殖器官同节段支配的穴位其激活下丘脑中GnRH相关神经元的作用最强。
Objective To study the influences of acupuncture in different acupoints on activities of hypothalamus GnRH-related neurons in female rats,compare the validity of different acupoints in body＇s different parts to the neuron activities,and provide experimental basis for the treatment of the diseases of propagative and incretory system with acupuncture.Methods The discharge of GnRH-related neurons in the medial preoptic area,arcuate nucleus and paraventricular nucleus of hypothalamus were recorded extracellularly by using glass microelectrode to study the difference of 15 acupoints in body＇s different parts in activation and activation intensity to neurons.Results The percentage of activated neuron discharge changes before and after acupuncture in 15 acupoints showed a descending order as follows：Zigong（EXCA1）, Guanyuan（CV4）,Daimai（GB26）,Sanyinjiao（SP6）,Erjia,Zusanli（ST36）,Xiaguan（ST7）, Guanyuanshu（BL26）,Shenshu（BL23）,Zhongwan（CV12）,Quchi（LI11）,Hegu（LI4）,Neiguan （PC6）,Ganshu（BL18） and Danzhong（CV17）.The acupuncture positions with obvious changes included low abdominal,hind limbs and lower back.The influence of acupuncture on inhibited neurons was as the same as that on activated ones.Conclusion Acupuncture can regulate effectively the activity of hypothalamus-hypophysis-gonadal endocrine axis,but the effect is different in different acupoints.The regulation effect of acupuncture in an acupoint is related closely to the spinal segment dominated position of the same acupoint.The acupoints with the same spinal segment domination of the genital organs have the largest activation on hypothalamus GnRH-related neurons.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2010年第3期191-195,199,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(No.2005CB523308)
