摘要
以８０％的冷甲醇浸提烟叶样品中的生长素（ＩＡＡ）、脱落酸（ＡＢＡ），通过控制ｐＨ值，经过多次液液分配并结合聚乙烯吡咯烷酮（ＰＶＰ）和Ｃ１８筒柱进一步分离纯化，用反相液相色谱检测烟叶成熟过程中内源ＩＡＡ、ＡＢＡ的动态变化。结果表明：下部叶叶色黄绿，黄色明显，主脉１／２～２／３白，支脉１／３白；中部叶叶色黄绿以黄为主至淡黄，主脉近全白，支脉近２／３白等外观特征出现时，叶片进入迅速衰老时期，超过上述时期的叶片应为过熟。
IAA and ABA in tobacco leaf samples were diffused with 80% cool methanol.By controlling pH value,after several times of liquid liquid partition and further separation and purification with PVP and C 18 column,changes of IAA and ABA during maturing were tested by reversed phase HPLC.The results suggest that tobacco leaves enter quick senescence period when the following external property appears:greenish yellow lower leaves are notably yellow with 1/2￣2/3 of midrib and 1/3 of lateral vein in white,greenish yellow middle leaves turn mainly yellow to light yellow,with midrib nearly all in white and 2/3 of lateral vein in white.The leaves exceeding that period are over mature.
出处
《中国烟草科学》
CSCD
1998年第4期41-43,共3页
Chinese Tobacco Science
