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髓样分化蛋白-2在转导内毒素信号中的作用及临床意义 被引量：2

Role and Clinical Significance of Myeloid Differentiation Protein-2 in Endotoxin Signal Transduction
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摘要 在脂多糖(LPS)诱导的细胞信号转导过程中,髓样分化蛋白-2(MD-2)与LPS和Toll样受体4(TLR4)分别结合,发挥桥梁分子的作用,介导TLR4对LPS的识别。LPS是与MD-2结合,而不是TLR4,没有MD-2的参与,细胞也不能发生反应或反应微弱。MD-2可被分泌到血浆中,形成可溶性的MD-2,对只含有TLR4而无MD-2的细胞进行远程调控。表明MD-2在转导内毒素信号中具有重要作用。MD-2具有分子质量小、核酸片段短、便于调控等特点,是极具潜力的新型抗炎靶点。 Myeloid differentiation protein-2（MD-2）can separately and simultaneously bind lipopolysaccharide（LPS）and toll-like receptor 4（TLR4）has been shown to play critical roles in mediated recognition responses to LPS by TLR4 and signal transduction induced by LPS.MD-2 can be bound by LPS,not TLR4.The cells have no responsiveness or weak responsiveness to LPS without MD-2.MD-2 can be secreted into blood plasma,formed soluble MD-2 and remotely regulated cells that contained TLR4 without MD-2.MD-2 has been shown to play important roles in endotoxin signal transduction.MD-2 is a small molecular,short nucleic acid fragment,easily regulated should become a new potential anti-inflammatory target.
作者 袁西华 陈昌辉
机构地区 四川省医学科学院 四川省人民医院儿科
出处 《实用儿科临床杂志》 CAS CSCD 北大核心 2010年第6期451-454,共4页 Journal of Applied Clinical Pediatrics
基金 四川省科技厅科研基金项目(05JY029-054-2)
关键词 髓样分化蛋白-2 脂多糖 TOLL样受体4 信号转导 感染 婴儿 新生 myeloid differentiation protein-2 lipopolysaccharide toll-like receptor 4 signal transduction infection infant newborn
分类号 R362 [医药卫生—病理学]
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参考文献29

  • 1徐发良,李磊.髓样分化蛋白-2在天然免疫中的作用[J].生理科学进展,2004,35(2):139-142. 被引量：5
  • 2Shimazu R, Akashi S, Ogata H,et al. MD - 2, a Molecule that confers lipopolysaccharide responsiveness on toll - like receptor 4 [ J ]. J Exp Med, 1999,189 ( 11 ) : 1777 - 1782.
  • 3Visintin A, Halmen KA, Latz E, et al. Pharmacological inhibition of endotoxin responses is achieved by targeting the TLR4 coreceptor, MD -2 [ J]. J Immunol,2005,175 ( 10 ) :6465 - 6472.
  • 4Prohinar P, Re F, Widstrom R, et al. Specific high affinity interactions of endotoxin. Protein complexes with toll -like receptor 4 ectodomain [ J ]. J Biol Chem,2007,282(2) : 1010 - 1017.
  • 5Mancek - Keber M,Jeraia R. Structural similarity between the hydrophobic fluorescent probe and lipid A as a hgand of MD -2[ J]. FASEB J, 2006,20( 11 ) :1836 - 1842.
  • 6Hyakusima N, Mitsuzawa H, Nishitani C. Interaction of soluble form of recombinant extracellular TLR4 domain with MD -2 enables lpopolysaccharide binding and attenuates TLR4 - mediated signaling[ J]. J Immunol,2004,173 ( 11 ) :6949 - 6954.
  • 7Shin HJ,Lee H, Park JD, et al. Kinetics of binding of LPS to recombinant CD14 ,TLR4 ,and MD - 2 proteins [ J ]. Mol Cells, 2007,24 ( 1 ) : 119 - 124.
  • 8Zimmer SM,Zughaier SM, Tzeng YL,et al. Human MD - 2 discrimination of meningococcal lipid A structures and activation of TLR4[ J]. Glycobiology, 2007,17 (8) :847 - 856.
  • 9Ohto U, Fukase K, Miyake K,et al. Crystal structures of human MD - 2 and its complex with antiendotoxic lipidiva [ J ]. Science, 2007,316 (5831) : 1632 - 1634.
  • 10Kobayashi M, Saitoh S, Tanimura N, et al. Regulatory roles for MD -2 and TLR4 in ligand - induced receptor clustering [ J ]. J Immunol, 2006,176 (10) :6211 - 6218.

二级参考文献42

  • 1刘靖华,赵克森.TLR与天然免疫反应[J].免疫学杂志,2001,17(z1):17-20. 被引量：9
  • 2熊建琼,朱佩芳,王正国,蒋建新.髓样分化蛋白2在内毒素/脂多糖激活人脐静脉内皮细胞中作用的实验研究[J].中华烧伤杂志,2005,21(2):97-99. 被引量：5
  • 3Medzhitov R,Preston-Hurlburt P,Janeway CA Jr.A human homologue of the drosophila Toll protein signals activation of adaptive immunity.Nature,1997,388:394-397.
  • 4Miyake K.Endotoxin recognition molecules,Toll-like receptor 4-MD-2.Semin Immunol,2004,16:11-16.
  • 5Visintin A,Mazzoni A,Spitzer JA,et al.Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4.PNAS,2001,98:12156-12161.
  • 6Nagai Y,Akashi S,Nagafuku M,et al.Essential role of MD-2 in LPS responsiveness and TLR4 distribution.Nat Immunol,2002,3:667-672.
  • 7Fitzgerald KA,Rowe DC,Golenbock DT.Endotoxin recognition and signal transduction by the TLR4/MD2-complex[J].Microbes Infect,2004,6(15):1361-7.
  • 8Miller SI,Ernst RK,Bader MW.LPS,TLR4 and infectious disease diversity[J].Nat Rev Microbiol,2005,3(1):36-46.
  • 9Jares-Erijman EA,Jovin TM.FRET imaging[J].Nat biotechnol,2003,21(11):1387-95.
  • 10Zal T,Gascoigne NR.Using live FRET imaging to reveal early protein-protein interactions during T cell activation[J].Curr Opin Immunol,2004,16(5):674-83.

共引文献24

同被引文献35

  • 1张金萍,陈超,杨毅.Toll样受体2和4在新生儿感染时的变化及其临床意义[J].中华儿科杂志,2007,45(2):130-133. 被引量：15
  • 2Wenzel J, Held C, Palmisano R, et al. Measurement of TLR - induced macrnphage spreading by automated image analysis: Differential role of Myd88 and MAPK in early and late responses[J]. Front Ph~siol,2011, 2: 71.
  • 3Jeong E,Lee JY. Intrinsic and extrinsic regulation of innate immune re- ceptor[ J]. Yonsei Med J,2011,52(3) : 379 -392.
  • 4Ohnishi B ,Tochio H, Kato Z, et al. Structural basis for the muhiple in- teractions of the MyDgg TIR domain in TLR4 signaling[ J]. Proc Natl Acad Sci U S A ,2009,106(25 ) : 10260 - 10265.
  • 5Chaudhary A, Ganguly K, Cabantous S, et al. The brueella TIR - like protein TepB interacts with the death domain of MyD88 [J]. Bioehem Biophys Res Commun,2012,417 ( 1 ) :299 - 304.
  • 6Avbelj M, Horvat S,Jerala R. The role of intermediary domain of MyD88 in cell activalion and therapeutic inhibition of TLIRs [ J ]. J lmmunol, 2011,187 ( 5 ) : 2394 - 2404.
  • 7Kawal T,Akira S. The role of pattern - recognition receptors in innate immunity : Update on Toll - like receptors [ J ]. Nat Immunol, 2010, 11 (5) : 373 -384.
  • 8Kawai T, Adachi O, Ogawa T,et al. Unresponsiveness of MyD88 - defi- cient mice to endotoxin[ J]. Immunity,1999,11 ( 1 ) : 115 - 122.
  • 9Valkov E, Stamp A, Dimaio F, et al. Crystal structure of Toll - like re- ceptor adaptor MAIJTIRAP reveals the molecular basis for signal trans- duction and disease protection[ J]. Proc Natl Acad Sci U S A ,2011,108 (36) : 14879 - 14884.
  • 10Capelluto DG. Tollip: A multitasking protein in innate immunity and protein trafficking[ J~. Microbes Infect,2OI2,1d(2) : 140 - 147.

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实用儿科临床杂志
2010年 第6期

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