摘要
本文采用蒸馏水沉淀法提取了德国小蠊卵黄蛋白，免疫家兔，并将所得抗血清经雄虫血淋巴吸收，获得抗卵黄蛋白特异抗体，用ＤＥＡＥ－纤维素批次法纯化抗体ＩｇＧ，并用辣根过氧化物酶标记，建立ＥＬＩＳＡ直接法测定卵黄蛋白含量的方法。
The present paper is the result of development of an ELISA for measurement of vitellin content in Blattella germanica, the vitellin of Blattella germanica was extraction with 0 4M NaCl and subsequent precipitation in cold distilled water. A ployclonal antibody against vitellin was prepared in male rabbits and made the specific antibody for vitellin by absorption with male cockroach haemolymph. The ELISA was established to quantify vitellogenin of the insect through purifying and conjugating the IgG to HRP.
出处
《寄生虫与医学昆虫学报》
CAS
1998年第4期246-252,共7页
Acta Parasitologica et Medica Entomologica Sinica
关键词
德国小蠊
卵黄蛋白
酶标抗体
ELISA
Vitellin\ \ ELISA\ \ Blattella germanica Enzyme labelled antibody
