摘要
为了解青霉胺对缺血后再灌注心肌损伤的影响，我们采用Ｌａｎｇｅｎｄｒｏｆ离体大鼠心脏灌注模型，先灌注１５ｍｉｎ后停止灌注，模拟缺血６０ｍｉｎ，然后再灌注６０ｍｉｎ。动物分为对照组及青霉胺处理组（３０ｍＭ）。分别测定缺血前及再灌注后心肌组织内三磷酸腺苷（ＡＴＰ）、谷胱甘肽（ＧＳＨ）及谷胱甘肽氧化酶（ＧＳＨ－Ｐｘ）的含量变化，再灌注过程中冠状动脉血流阻力及冠脉流出液中磷酸肌酸激酶（ＣＰＫ）的释放量。结果显示，青霉胺处理组再灌注后心肌组织的ＡＴＰ、ＧＳＨ、ＧＳＨ－Ｐｘ含量均明显高于对照心肌组织中的含量，而青霉胺处理组在再灌注过程中ＣＰＫ的总释放量及平均冠状血流动脉阻力明显低于对照组ＣＰＫ总释放量及平均冠状动脉血流阻力。提示青霉胺可以减轻缺血后再灌注的心肌的损伤。
To investigate the effects of penicillamine on postischemic myocardial reperfusion injury,we used Langendorff isolated rat heart as the experimental model in this study.The hearts were divided into control and penicillamine (30mM) treated group. After perfused for 15 min the hearts received a total globe ischemia simulated by a cessation of perfusion for 60 min and were reperfused for 60 min.The content of ATP,GSH and GSH Px in myocardia of baseline and after reperfusion and the CPK in coronary effluent and coronary flow resistance during reperfusion were examined.The results in control and penicillamine treated group were as following:ATP 1.0474±0.28 μmol/gww,1.7569 ±0.20 μmol/gww( P <0 05);GSH 51.86±0 72 μg/100 mgww,274.17±32.59 μg/100 mgww( P <0.01);GSH Px 1.90±0.18 μmol/min/gww,3.21±0.25 μmol/min/gww( P <0.01).The total CPK release and average coronary flow resistance in control and penicillamine treated group were 124.30±13.41 Iu/gww 22.40±3.92 IU/gww( P <0.01);8532.25±737.01 kPa.min/L, 2805±283.72 kPa.min/L( P < 0.01), respectively.Our findings suggested that penicillamine could attenuate the postischemic myocardial reperfusion injury.
出处
《中国应用生理学杂志》
CAS
CSCD
1998年第4期333-335,共3页
Chinese Journal of Applied Physiology
