摘要
在表面活性剂吐温－８０存在下，硒与硫氰酸盐和罗丹明Ｂ形成水溶性离子缔合物，分光光度法测定硒。缔合物在５９５ｎｍ处有最大吸收，硒量在０～５．０μｇ／２５ｍｌ范围内符合比尔定律，表观摩尔吸光系数为４．４２×１０５Ｌ·ｍｏｌ－１·ｃｍ－１。结合巯基棉分离富集技术，测定了尿硒，结果表明该法灵敏度高，选择性好。
An ion-associate complex was formed from the selenium,thiocyanate and Rbodamine B.The Maximum absorption of the complex is at 595nm,and its apparent molar absorptivity is 4.42×10 5 L·mol -1 ·cm -1 ,Beer's law is obeyed for 0～5.0μg/25ml of selenium.The proposecmethod has been used for the determination of selenium in urine by using sulfhydryl cotton.The results above shows that this method is good in selectivity and sensetivity.
出处
《微量元素与健康研究》
CAS
1998年第4期55-56,共2页
Studies of Trace Elements and Health
关键词
硒
硫氰酸盐
罗丹明B
尿
巯基棉
Selenium Thiocyanate Rhodamine B Urine Spectrophotometry
