摘要
探究不同浓度的激素对黄檗嫩茎腋芽丛生芽苗、芽苗生根和试管苗保存的影响。应用均匀设计法筛选黄檗嫩茎离体培养和种质试管保存各阶段最适合的培养基。最适合诱导嫩茎腋芽丛生芽苗的培养基为:N6+TDZ2.86mg/L+IAA0.03mg/L,诱导率为99.5%;生根培养基为:1/2N6+KT0.45mg/L+IBA0.10mg/L,生根率达97%以上;试管苗保存培养基为:N6+根皮苷2.65mg/L+KT0.15mg/L,在常温条件下,在试管内保存黄檗种质可达45个月以上,生长率仅达0.74%。试验结果表明,通过腋芽丛生和延缓生长的方法成功建立了黄檗的离体培养和种质试管保存体系。
Explored the effect of various hormones with different concentrations to induce multiple shoot clumps from axillary bud of Phellodendron amurense, root of shoots and germplasm peservation in vitro of the young stems of Phellodendron amurense Rupr.. Uniform design for screened the suitable medium compositions of in vitro cultured and germplasm peservation in vitro. N6＋TDZ 2.86 mg/L＋IAA 0.03 mg/L for axillary bud multiple shoot clumps from tender stem, the rate of induction was 99.5%; 1/2 N6＋KT 0.45 mg/L＋IBA 0.10 mg/L for rooting, the rate of rooting was more than 97%; N6＋phlorizin 2.65 mg/L＋KT0.15 mg/L for germplasm preservation in vitro for 45 months by the retarding of growing at normal temperature, the rate of growth was 0.74%. The experiment results showed that in vitro culture and germplasm conservation in vitro system of Phellodendron amurense have been successfully established by the methods of inducing axillary bud multiple shoot clumps and the retarding of growing.
出处
《中国农学通报》
CSCD
北大核心
2010年第9期255-258,共4页
Chinese Agricultural Science Bulletin
基金
国家科技部"国家科技攻关计划引导项目"资助项目(2005BA741C)
吉林省科技厅资助项目(200705C05)
关键词
黄檗
腋芽
种质试管保存
均匀设计
Phellodendron amurense Rupr.
axillary bud
germplasm peservation in vitro
uniform design
