摘要
总结了荧光探针技术在两亲分子有序组合体研究中的应用.在两亲分子有序组合体研究中,荧光技术的应用非常广泛.尤其是荧光探针技术的应用,为两亲分子有序组合体微观信息的获取提供了一个简单便利的渠道.根据荧光探针分子的荧光光谱,如最大发射波长、荧光强度及荧光寿命等参数,可以得到临界聚集浓度、微粘度、微极性等信息;根据探针的荧光猝灭,尤其是时间分辨荧光猝灭技术,可探测有序组合体的聚集数、聚集数变化相应的聚集体转化及表面电荷等信息.近年来发展起来的含荧光基团的两亲分子因其可以对聚集体微环境进行原位检测而引起了了广泛的关注.本文对上述几个方面的相关工作分别作了介绍.
In this paper,we review the application of fluorescent probe technology in self-assembly systems of amphiphile aqueous solutions.Fluorescence technology,especially fluorescence probe technology has been extensively employed to explore local information about various molecular assemblies.The following contents are covered：（1） The critical aggregate concentration,microviscosity,and micropolarity may be obtained from fluorescence parameters such as emission maxima,fluorescence intensity,and lifetime etc.（2） Probe quenching,especially,timeresolved fluorescence quenching （TRFQ） can give information about aggregation number and surface charge density which can be used as the indication on aggregate transition.（3） Fluorophore-labeled amphiphiles which can take part in the formation of aggregates reveal more precise information,so that an in situ investigation of local environments is possible.Moreover,fluorescence resonance energy transfer （FRET） and excited state proton transfer （ESPT） are also useful in this field.Therefore,fluorescence probe technology provides us with a simple and effective method to study organized amphiphiles systems.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
2010年第4期840-849,共10页
Acta Physico-Chimica Sinica
基金
国家自然科学基金(20873001,20633010,50821061)资助项目~~
