摘要
针对流式细胞术的核心之一——单细胞的液流驱动系统,提出了基于微流体数字化驱动-控制技术实现单细胞排列及输出的方法,分析了单细胞流的驱动机理,通过对流动室施加数字化可编码的高频驱动脉冲,细胞从连续的鞘液流中分割成单细胞流输出。设计了一种流式细胞仪核心部分石英微流动室,并采用石英玻璃精细加工而成。进行了人体外周血淋巴细胞的驱动实验研究,可通过荧光显微镜实时观测微通道内的单细胞流动情况,方法简单,操作方便。
The flow driving system of single cell is one of the key components of flow cytometer.The method of single cell arrangement was proposed based on the digitalization of microfluids driving and controlling technology,and the driving mechanism of single cell flow was analyzed.By way of encoded digital high frequency pulse imposed on flow chamber,cells from continuous sheath fluid were split into single cell.One quartz micro flow chamber of the core of flow cytometry fabricated by sophisticated glass-processing technology was designed.The driving experiment on the lymphocytes of human peripheral blood was conducted,and real-time observation of fluidic status of single cell was performed through the fluorescence microscope.The experiment showed that the entire system was compact and easy to operate.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2010年第4期949-954,共6页
CIESC Journal
基金
教育部博士学科点专项科研基金项目(20060288005)
江苏省自然科学基金项目(BK2007209)
国家自然科学基金项目(50975152)~~
关键词
流动室
微流体数字化
单细胞流
流式细胞术
flow chamber
digitalization of microfluids
single cell flow
flow cytometry
