摘要
目的观察新生大鼠海马齿状回的神经干细胞(NSCs)移植到阿尔茨海默病(AD)模型大鼠海马内的存活、迁移和分化。方法将Hoechst33258标记的NSCs植入AD模型大鼠海马,移植2、4和6w后,行Y迷宫检测大鼠的学习记忆能力,结合Hoechst33258标记和荧光免疫组化技术,分析NSCs在AD大鼠海马中的存活、迁移和分化。结果移植的NSCs在宿主脑内能够存活至少6w,沿海马回向两侧迁移,NSCs移植2w后,免疫荧光检测无明显神经丝蛋白-200(NF-200)、胶质纤维酸性蛋白(GFAP)、谷氨酸(Glu)、胆碱乙酰转移酶(ChAT)阳性反应,而移植后4、6w,则可见免疫阳性反应细胞。Y迷宫测试结果显示移植NSCs2、4、6w后大鼠学习、记忆能力明显得到恢复,与AD模型组比较有统计学意义(P<0.05),与正常对照组比较,差异无统计学意义(P>0.05)。结论自新生大鼠海马齿状回分离培养的NSCs在AD模型大鼠海马内能够存活、迁移并分化为胆碱能、谷氨酸能神经元及神经胶质细胞。
Objective To observe the survival,migration and differentiation of neural stem cells （NSCs） from rat hippocampus after transplantation into the AD rat hippocampus.Methods After labeled with Hoechst33258,NSCs were grafted into the hippocampus of AD rats.2,4 or 6 weeks post transplantation,the behavioral testing in the rats were performed using Y-maze trial.Then double-label technique （Hoechst33258-label technique and immunofluorescence technique） was applied to detect the survival,migration and differentiation of NSCs.Results The grafted neural stem cells survived well and moved to opposite directions along hippocampal gyrus.2 weeks after transplantation,grafted NSCs did not express nerve fibrosis-200 （NF-200）,growth factor acid protein （GFAP）,cholinergic aminotransase （ChAT） or glutamate （Glu） positive staining of immunofluorescence,while after transplantation for 4,6 weeks,in the graft,above-mentioned positive cells were expressed.Y-maze trial results showed that,as comparing with AD rats,the ability of learning and memory in 2,4,6-week-transplantation groups enhanced obviously（P〈0.05）.But there were no significant difference between transplantation groups and normal groups（P〈0.05）.Conclusions The grafted NSCs may survive,migrate and differentiate into astrocytes and neurons,especially cholinergic neurons and glutamate neurons.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2010年第6期779-781,共3页
Chinese Journal of Gerontology
基金
河南省医学创新人才基金资助项目(2002115)
关键词
阿尔茨海默病
神经干细胞
移植
分化
Alzheimer＇s disease
Neural stem cells
Transplantation
Differentiation
