摘要
利用JMP和Box-Behnken响应曲面设计(RSM),研究了培养条件对猕猴桃酒酵母WF25增殖作用的影响。研究结果表明:温度、装液量、转速、初始pH和培养时间对猕猴桃酒酵母WF25细胞的增殖均有极显著的影响。当培养条件为温度28.5℃、初始pH4.5、转速175r/min、装液量65mL和时间30h时,生物量的预测值可达3.06352×108个/mL。
The effects of cultivated conditions on WF25 proliferation role of kiwifruit wine yeast were studied.The result of JMP and Box-Behnken response surface methodology （RSM） indicated： temperature, volume of medium, rotated speed,initial pH and time had very significant influence.Optimum cultivated conditions of cell proliferation were temperature 28.5%, initial pH4.5, rotated speed 175r/min, volume of medium 65mL and time 30h, the prediction value of biomass reach to 3.06352 ×10^8/mL.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第4期183-186,共4页
Science and Technology of Food Industry
基金
国家西部专项(2001BA901A19)
关键词
猕猴桃酒酵母
培养条件
响应曲面
kiwifruit wine yeast
cultivated conditions
response surface methodology（RSM）
