摘要
目的:探讨氯化锂预处理对老年大鼠腹部探查术后空间记忆能力的影响。方法:24只18月龄SD大鼠随机分为3组,即对照组(C)、手术组(O)和氯化锂预处理手术组(L/O),每组8只。L/O组大鼠腹腔注射氯化锂2mmol/kg,每天1次,连续7天,O组和C组大鼠给予等量生理盐水,除C组外全部大鼠于第8天建立腹部探查手术模型,术后24h用Morris水迷宫测试大鼠的空间记忆能力。结果:虽然O组或L/O组大鼠的逃避潜伏期比C组大鼠显著增加(P<0.05),但是L/O组大鼠的逃避潜伏期较O组大鼠明显改善(P<0.05);C组大鼠的游泳距离显著短于O组或L/O组(P<0.05),其中L/O组大鼠的游泳距离显著短于O组(P<0.05)。结论:老年大鼠行腹部探查术后可发生空间记忆能力的下降,氯化锂预处理后可明显减缓这些变化,提示氯化锂预处理具有防治老年大鼠术后空间记忆能力下降的效应。
Objective：To study the effect of lithium chloride pretreatment on spatial memory in aged rats’ hippocampuses after exploratory laparotomy. Methods：Twenty-four male SD rats,aged 18 month,were divided into three groups randomly （n=8 each）： control group （C）,operation group （O） and lithium chloride group （L / O）. The rats in group L / O were injected with 2 mmol / kg lithium chloride into their peritoneal once a day and continued seven days,while the rats in the other two groups were injected with same volume of normal saline. All rats were established exploratory laparotomy model on eighth day. After 24 h,the rats’ spatial memory was measured by Morris test. Results：The latency of group C was lower than that of group L / O or group O（P〈0.05）;the latency of group L / O was significantly lower than that of group O（P〈0.05）;the swimming distance of group C was significantly shorter than that of group L / O or group O（P〈0.05）;the swimming distance of group L / O was significantly shorter than that of group O（P〈0.05）. Conclusion：Spatial memory was injured after exploratory laparotomy in aged rats. However,lithium chloride pretreatment could obviously improve these changes. It is suggested that lithium chloride pretreatment has potential effects for spatial memory decrease in aged rats after exploratory laparotomy.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期380-382,408,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅医学重点人才项目(2001H58)
关键词
氯化锂
老年大鼠
术后认知功能障碍
lithium chloride
aged rats
postoperative cognitive dysfunction
