摘要
目的探讨糖尿病性勃起功能障碍(ED)大鼠阴茎海绵体平滑肌细胞(CCSM)的体外培养方法,建立糖尿病性ED大鼠CCSM体外培养模型,为糖尿病性ED的研究奠定基础。方法建立糖尿病性ED大鼠动物模型,用改良组织块贴壁法体外培养CCSM并行免疫组织化学染色鉴定,观察细胞形态和生长情况。结果糖尿病性ED大鼠造模成功;用改良组织块法培养糖尿病ED大鼠CCSM,3d后可见细胞游出,16～18d细胞长成单层,传代后细胞增殖旺盛,呈"峰-谷"样生长。培养的细胞经α-SM-actin和结蛋白免疫组化染色鉴定为平滑肌细胞,糖尿病性ED大鼠CCSM体外培养模型建立成功。结论改良组织块贴壁法建立糖尿病ED大鼠CCSM体外培养模型简便、稳定;糖尿病ED大鼠CCSM较正常CCSM增殖速度快,在光镜下形态与正常CCSM差别不明显。
Objective To investigate the method for culturing corpus cavernosum smooth muscle cells（CCSMs） derived from diabetic rats with erectile dysfunction（ED） for the study of ED caused by diabetes.Methods CCSMs were isolated from the corpus cavernosum of diabetic rats with ED and cultured using a modified method of adherent tissue culture.The cultured cells were identified by immunohistochemistry and the cell morphology and proliferation were observed.Results The primary culture of CCSM was performed successfully,and the cells were seen to migrate from the small tissue pieces 3 days later,reaching nearly confluence in 16-18 days.A typical ＂hill-valley＂ growth pattern was noted in the cell passaging.Immunohistochemical staining for α-smooth muscle actin（α-SM-actin） and desmin yielded positive results in the cells.Conclusion The modified method for adherent tissue culture is convenient and reliable in establishing the in vitro cell culture model of CCSMs from diabetic rats with ED,and the cultured CCSMs display a faster proliferation than normal CCSMs.No obvious differences in the cell morphology can be found between diabetic and normal CCSMs under light microscope.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第2期351-354,共4页
Journal of Southern Medical University
基金
南方医院院长基金面上项目(2008B014)
关键词
糖尿病
勃起功能障碍
阴茎海绵体平滑肌
细胞培养
疾病模型
动物
diabetes
erectile dysfunction
corpus cavernosum smooth muscle cells
cell culture
disease model
animals
