摘要
目的采用骨室种植体体内检测技术探讨骨周围骨骼肌收缩对骨毛细血管通透性的影响。方法30只雌性健康新西兰大白兔(6～10月,3～3.5kg)随机分为两组,每组15只,所有动物均于右侧胫骨植入骨室种植体。实验组于术后第2周开始行经皮神经肌肉电刺激,刺激腓肠肌收缩,频率4Hz,1h/d,每周6日;对照组无刺激。术后3周起,每周通过骨室种植体行体内显微观察至第10周,光镜下通过荧光染料FITC和RITC观察血管生长情况,运用Metamorph图像分析软件进行分析。结果实验组由经皮神经肌肉电刺激引起腓肠肌收缩,随着腓肠肌收缩时间延长,实验组毛细血管通透性显著大于对照组。结论应用骨室种植体体内检测技术能够较好在体检测骨内毛细血管通透性,骨骼肌收缩能提高骨毛细血管通透性。
Objective To assess the effect of muscle contraction on bone capillary permeability.Methods Thrity female New Zealand white rabbits were randomly divided into the experimental and control groups（n=15）.The bone chambers were implanted medially into the right tibiae of the rabbit,and in the experimental group,the rabbits were stimulated by transcutaneous electrical nerve stimulator（TENS） at 4 Hz（1 h/day,6 days/week） starting from week 2.From week 3,intravital microscopic observation was carried out weekly till week 10,and the vasculature in the tibiae was observed under fluorescent microscope with FITC and RITC staining.All the images were analyzed by Metamorph program.Result The bone capillary permeability increased significantly in the experimental group.Conclusion Skeletal muscle contraction can increase the capillary permeability.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第2期295-297,共3页
Journal of Southern Medical University
基金
广东省科技计划项目(2007B010600051)
国家自然科学基金(30872638)
