摘要
利用真菌β-微管蛋白基因的保守序列和小麦条锈菌(Puccinia striiformis f.sp.tritici)、叶锈菌(Puccinia tri-ticina)的SCAR标记引物,对锈菌孢子与感病小麦叶片总DNA进行复合PCR检测,扩增获得了小麦条锈菌171bp和叶锈菌226 bp的特异性DNA片段,其检测灵敏度可达到50μg.L-1模板DNA浓度水平.在此基础上,可进一步制备小麦锈菌不同种的分子诊断、检测试剂盒.
In this research,a multiplex PCR analysis system was built successfully.The 171 bp specific DNA fragment of Puccinia striiformis f.sp.tritici and the 226 bp specific DNA fragment of Puccinia triticina were amplified from the corresponding urediospores and the infected wheat leaves.The detection sensitivity of this multiplex PCR was 50 μg·L-1 template DNA.On this basis,a high-through kit can be further developed for the rapid diagnosis and detection of wheat rust fungi.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2010年第1期74-77,90,共5页
Journal of Henan Agricultural University
基金
国家"863"计划课题(2006AA10Z429)
中国博士后基金(20060400539)
国家科技支撑计划课题(2006BAD08A14)
河南省科技攻关计划(082102170024
092300410092)
