摘要
[目的]对成都西岭雪山土壤中粘细菌进行分离鉴定,了解其发酵产物粗提物的抗菌活性。[方法]以滤纸为唯一碳源,得到1株粘细菌CMC0606;通过观察其形态及16SrDNA克隆与测序进行菌种鉴定;向其发酵液中加入大孔吸附树脂XAD-16,收获发酵产物粗提物。[结果]菌株CMC0606被鉴定为珊瑚状珊瑚球菌Corallococcus coralloides CMC0606;其发酵产物粗提物对枯草芽孢杆菌、金黄色葡萄球菌及辣椒疫霉具有抑制作用。[结论]菌株CMC0606具有抗细菌和抗真菌的活性,尤其对植物致病菌辣椒疫霉抑制作用显著,具有潜在的研究和开发价值。
[Objective] Myxobacteria in Xiling Snow Mountain of Chengdu was isolated and purified,which aimed to learn the antibiotic activity of crude extracts. [Method] With filter paper as the sole carbon source,a Myxobacteria CMC0606 was obtained; through observing its morphology,16S rDNA cloning and sequencing,the bacteria was identified; adding XAD-16 into fermentation broth,crude extracts of fermentation products were obtained. [Result] CMC0606 was identified as Corallococcus coralloides; the crude extracts of its fermentation products had inhibition effects on Bacillus subtilis,Staphylococcus aureus and Phytophthora capsici Leonian. [Conclusion] CMC0606 has activity of anti-bacterial and anti-fungal,especially for plant pathogens Phytophthora capsici.
出处
《安徽农业科学》
CAS
北大核心
2010年第9期4445-4446,4449,共3页
Journal of Anhui Agricultural Sciences
基金
四川省科技厅应用基础研究项目(05JY029-118)
关键词
粘细菌
珊瑚球菌
粗提物
抗菌活性
Myxobacteria
Corallococcus coralloides
Crude extract
Antibiotic activity
