摘要
目的：为了研究硫代反义寡核苷酸（ASON）的抗乙型肝炎病毒（HBV）作用。方法：以2.2．15细胞为靶细胞，在polyA增加信号序列设计了1段ASON，用酶联免疫吸附法检测了作用细胞的HBV病毒抗原的分泌情况。结果：ASON在每天给予2μmol／L的浓度情况下能抑制77％的HBsAg和71％HBeAg的产生，无关序列的寡核苷酸无明显抑制作用，同时未见ASON的细胞毒作用。结论：提示ASON可能成为抗HBV治疗的新手段。
objective: To study the iwhibitory effect of antisense olignnuclcoides on the expression of hepatitis B virus surface antigen. Methods: Human hepatoma cell line 2 .2 .15 harbors cloned HBV DNA and produces and secretes HBV surface antigen and e antigen to the medium. A synthetic antisense oligonucleotide complementary to poly A addition signal region was synthesized and its ability to block the gene expression was tested. Results: The antisense olignnucleotide. at a concentration of 2μmol/L perday could inhibit 77% of the HBsAg and 71% of HBeAg produced. An unrelated oligonucleotide sequence showed no effect, Oligonucleotides had no cytotoxicity. Conclusion: These results demonstrate the antiviral effect or antisense oligonucleotide in vitro and exemplity their potential for human antiviral therapy.
出处
《泸州医学院学报》
1998年第4期272-275,共4页
Journal of Luzhou Medical College
