摘要
目的研究不同卵巢反应性的种植窗期子宫内膜组织中基质细胞衍生因子-1α(SDF-1α)及其受体CXCR4的表达。方法收集种植窗期子宫内膜组织(n=17),根据卵巢反应性分为卵巢高反应组(n=6)、卵巢中等反应组(n=6)和正常对照组(n=5)。应用RT-PCR、Real-time PCR、免疫组织化学染色和Western blotting方法,检测并比较各组种植窗期子宫内膜组织SDF-1α/CXCR4 mRNA和蛋白表达。结果免疫组织化学染色显示,CXCR4在种植窗期子宫内膜组织中高表达;SDF-1α在内膜腺体和腔上皮细胞中表达,而在间质细胞中不表达。各组子宫内膜组织SDF-1α/CXCR4 mRNA和蛋白表达比较,差异均无统计学意义(P>0.05)。结论高甾体激素对种植窗期子宫内膜趋化因子SDF-1α及其受体CXCR4的表达无明显影响。提示SDF-1α/CXCR4可能不参与甾体激素对种植窗期CD56^(bright)CD16^-NK细胞募集的调控。
Objective To investigate the expression of stromal cell-derived factor-1α （SDF-1 α） and CXCR4 under different ovarian responses in human endometria of peri-implantation phase. Methods Endometria of peri-implantation phase （n = 17） were collected, and were divided into moderate ovarian response group （n = 6）, high ovarian response group （n = 6） and normal control group （n = 5）. The expression of SDF-1α/CXCR4 mRNA and protein in endometria of peri-implantation phase was detected and analysed by RT-PCR, Real-time PCR, immunohistochemistry and Western blotting. Results It was revealed by immunohistochemistry that CXCR4 was highly expressed in endornetrial tissues, whereas SDF-1α was confined to glandular and celomic epithelial cells instead of stromal ceils. There was no significant difference in the expression of SDF- 1α/CXCR4 mRNA and protein in human endometria among each group （P 〉 0. 05）. Conclusion High levels of steroid hormone do not affect expression of SDF-1 α/CXCR4 in human endometria of peri-implantation phase, indicating that SDF- 1α/CXCR4 may not participate in the recruitment of CD56^bright CD16 - NK cells to endometria by steroid hormone.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第3期305-309,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
上海市教委创新项目(06BZ051)~~
