摘要
本研究分析了红花清除自由基的能力,并探讨了有效提取其抗氧化成分的方法,旨在为红花的保健效用及其开发利用提供基本资料。以红花为试材,通过超声波、过滤、离心等方式,采用水、50%乙醇、70%乙醇、90%乙醇、95%乙醇、无水乙醇、丙酮、乙酸乙酯等溶剂提取抗氧化成分,应用水杨酸法测定其清除羟自由基的能力,应用光照核黄素法测定其对超氧阴离子自由基的清除作用。结果表明,通过超声波和离心结合的方式,利用95%乙醇对红花进行提取,提取液对羟自由基的清除作用最佳,清除率(98.31%)高于绿茶(81.85%)和VE(57.17%),与VC(99.60%)相当。同时,红花提取液对超氧阴离子自由基也有一定的清除作用,以70%乙醇对红花进行提取对超氧阴离子自由基的清除效果为佳。
In order to provide some basic information for the health care effect and the utilization of safflower （Carthamus tinctorius L.）, the flee radical scavenging capacity and the effective methods for antioxidant components extraction were studied. The safflower was used as the material, and the ultrasonic wave, filtration and centrifugation were employed. The solvents used were water, 50% alcohol, 70% alcohol, 90% alcohol, 95% alcohol, absolute alcohol, acetone, and ethyl acetate. The hydroxyl radical scavenging capacity and superoxide anion radical scavenging capacity of safflower extracts were measured using salicylic acid method aud riboflavin light treatment method, respectively. The combination of ultrasonic wave and centdfugation, with 95% alcohol as solvent was found as the best method for antioxidant components extraction from safflower. In this means, the scavenge rate was 98.31%, which was significantly higher than that of green tea （81,85%） and VE （57.17%）, respectively, although similar to that of Vc （99.60%）. The superoxide anion radical scavenging capacity of safflower was also found and 70% alcohol was believed as the best solvent for this purpose.
出处
《天津农学院学报》
CAS
2010年第1期6-9,共4页
Journal of Tianjin Agricultural University
基金
天津市科技支撑计划重点项目"红花种质资源利用与耐盐高产栽培技术研究"(08ZCKFNC01800)
天津市农业科技成果转化与推广项目"利用耐盐经济植物改良盐碱地技术示范"(0702150)
天津农学院科技发展基金项目"红花生物活性物质提取及功能分析"(2008D006)
关键词
红花
清除自由基能力
羟自由基
超氧阴离子自由基
抗氧化活性
Carthamus tinctorius
free radical scavenging capacity
hydroxyl radical
superoxide anion radical
antioxidant activity
