摘要
运用荧光显微技术,从2006年7月~2007年10月,分季度采样,对河北北戴河和天津沿岸海域的浮游细菌和病毒进行了调查。结果表明,浮游细菌和病毒具有明显的时空分布特点,细菌丰度超过10^6/mL,病毒丰度超过10^7/mL。病毒总数与细菌总数之比VBR为1.75~26.51,平均为6.22。运用超离心和电子显微镜对2007年春季样品进行检测,发现被病毒感染的细菌总频度为3%~5%,引起的细菌死亡率为28%~55%,平均为41.5%。揭示浮游病毒感染是浮游细菌死亡的重要原因,同时病毒在海洋系统物质循环和能量流动中扮演重要角色。
Virioplankton is considered to be an abundant and dynamic component that can regulate the biomass production and species composition of heterophic bacteria in aquatic ecosystems. Virioplankton and bacterioplankton abundance were measured by epifluorescence microscopy (EM) direct counts using SYBR Green I stain. Virioplankton abundance detected was much more than those of bacterioplankton. The abundance ratio of viruses to bacteria (VBR) was 1.75 -26.51, and the average was 6.22. Bacteria were harvested directly onto Fomvarcoated, 200-mesh electron-microscope grids by ultracentrifugation and the frequency of visibly infected cells (FVIC) was got by transmission electron microscopy (TEM). FVIC ranged from 3 to 5 % in the samples in Spring 2007,and the average of estimated viral mortality of bacteria (VMB) was 41.5 % (28 % -55 % ) , indicating that viruses could be significant contributors to bacterial losses in coastal areas of Hebei province.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2010年第2期187-190,共4页
Marine Environmental Science
基金
国家自然科学基金(40906082)
国家海洋局青年基金(2010111)
国家海洋局近岸海域生态环境重点实验室开放研究基金(200903)
我国近海海洋综合调查与评价专项项目(908-HB-02)
国家海洋局海洋生态与海洋生物地球化学重点实验室开放基金(LMEB200604)
国家863项目(2006AA09Z162,2006AA09Z170)