摘要
采用MPN-Griess和MPN-PCR两种方法，于2007年春季（5月份）、夏季（8月份）和秋季（11月份）分3个航次，对辽河口区域沉积物中的硝化细菌进行了计数，并对硝化细菌在时间和空间上的分布进行了定量分析。结果表明，MPN—PCR方法要灵敏于MPN-Griess方法10—100倍，且重复性好，能够更快速、更准确的测定硝化细菌的数量；辽河口沉积物中硝化细菌的数量有明显的季节差异，分布趋势为春季最高、夏季最低；空间水平分布趋势为：从河口上游至深海区，硝化细菌数量逐渐降低。说明硝化细菌数量的时空分布与环境温度和沉积物中N的含量有关。
Nitrobacteria in the sediment in Liaohe estuarine were investigated by MPN-Griess and MPN-PCR in spring （ May）, summer （Aug.） and autumn （ Nov. ） of 2007. The summarization of the results on temporal and spatial distribution of nitrobacteria in sediment was done. It is found that MPN-PCR is 10 0 100 times more sensitive than MPN-Griess and MPN-PCR characteristic of good repeatability, high test speed and accuracy in nitrobacteria test. The population of nitrobacteria in the sediment of Liaohe Estuary showed the obvious seasonal variation, and peaking was in spring and bottoming in summer. The spatial distribution of nitrobacteria in the sediment was gradually decreased from the estuarine upstream into the deep water. The studies also showed that the number of nitrobacteria on temporal and spatial distribution is related with temperature and nitrogen content in the sediments.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2010年第2期174-178,共5页
Marine Environmental Science
基金
国家自然科学基金资助项目(40676060)
