摘要
目的探索雌二醇水平与大鼠唾液腺之间的关系。方法 32只雌性Wister大鼠随机分为4组:对照组、假手术组、去势组和去势并给予雌激素组。摘除雌大鼠卵巢形成绝经期大鼠模型,通过检测血清雌二醇水平,观察各组大鼠唾液腺组织的HE切片和Cu,Zn-SOD的表达量来综合分析雌二醇水平降低对鼠唾液腺的影响。结果与对照组、假手术组和去势并给予雌激素组相比,去势组雌二醇水平明显降低(P<0.01)。组织形态学变化发现,去势并给予雌激素组腮腺腺泡发生空泡性变性;颌下腺浆液性腺导管上皮有轻度嗜酸性变化,管腔变小;舌下腺无明显变化。而去势组更为严重。免疫组化结果显示对照组和假手术组的腮腺组织中Cu,Zn-SOD呈现强阳性;颌下腺的浆液性腺导管上皮呈现强阳性,舌下腺为弱阳性。去势组和去势并给予雌激素组表达弱于对照组和假手术组,去势组表达最弱。结论雌激素水平降低对唾液腺组织有影响,而Cu,Zn-SOD参与了保护唾液腺组织的正常结构的过程。
Objective In this research,the relationship between blood estrogen level and salivary glands of female rat was studied. Methods Thirty - two female Wister rats were randomly divided into four groups ： control group, sham operation group,ovariectomized and injected estradiol benzoate group and ovariectomized group . Menopausal rat model was established by ovariectomy. By testing E2, histological examination and examing Cu, Zn -SOD expression through immunochemical staining, we studied the effects of estrogen decreasing on female rat salivary. Results Compared with control group ,sham operation group,ovariectomized and injected estradiol benzoate group ,a significant decrease in scrum estrogen level was observed in ovariectomized group（ P 〈 0.01 ）. By observation of histomorphology, the ovarieetomized and injected estradiol benzoate group were found that there was vacuolar degeneratio in the parotid acinar and the feweosinophilic changes were found in the serous submandibular gland duct epithelial and the lumen was smaller. There was no clear change in the sublingual gland and there were more obvious changes in the ovariectomized group. The results of immunohistochemistry showed that the positive Cu, Zn -SOD was found in control group and sham operation group. The positive Cu, Zn -SOD in groups with estrogen infusion after ovariectomy presented stronger than group with only ovariectomy. Conclusion Our results suggested that estrogen and Cu, Zn -SOD could suppress histologically changes in salivary glands （parotid, submandibular and sublingual） of female rat ,and estrogen could have influence on the production.
出处
《医学研究杂志》
2010年第3期56-58,143,共4页
Journal of Medical Research
基金
黑龙江省教育厅科学技术研究项目(11541353)
