摘要
本文用ELISA、细胞培养、RT-PCR方法研究哮喘患者的血浆和单个核细胞IL-10的水平及其与IgE的关系。结果显示32例哮喘患者血浆、细胞培养上清IL-10值和外周血单个核细胞IL-10转录水平均比20例正常人明显降低,血浆IL-10降低组不IgE明显高于血浆IL-10正常组。IL-10是一种抗炎因子,过敏性哮喘患者IL-10浓度降低说明其对过敏性炎症的抑制能力减弱,与哮喘的发病有关。
In order to study the role of IL-10 in asthmatics and relation with IgE, IL-10 was detected in 32 allergic asthmatics using ELISA, cell culture and RT-PCR. Comparing with those of 20 health controls. The result showed, IL-10 in plasma and cell culture supernatants were significantly lower in asthmatic groups than in control groups. As shown by RT-PCR, the expression of IL-10 mRNA in PBMC was lower in asthmatic groups than in control groups, plasma IgE value in lower IL-10 group were significantly higher than in normal IL-10 group. The results demonstrated that IL-10 may play an important role in the pathogenisis of allergic asthma.
出处
《上海免疫学杂志》
CSCD
北大核心
1998年第6期358-360,共3页
Shanghai Journal of Immunology
基金
国家自然科学基金(39500063)
卫生部基金(96-2-285)资助项目
