摘要
为了给白三叶草的选育和资源利用提供理论依据,采用聚丙烯酰胺凝胶电泳的方法,对来自不同地区的16份白三叶草(Trifoliumrepens L.)种质资源进行酯酶(EST)和过氧化物酶(POD)同工酶检测,并对检测到的5个等位酶位点的图谱进行分析,再根据各种质资源间遗传相似度及聚类结果进行分析。结果表明:白三叶草各种质资源间有较高的遗传多样性;16份白三叶草种质资源在相似系数为0.71的水平上可以聚为三类,大部分地理区域临近的种质材料首先聚在一起。说明他们有较近的亲缘关系。
The esterase （EST） isozyme and peroxidase （POD） isozyme of 16 Trifolium repens germplasm resources from different regions were determined by polyarcylamide gel electrophoresis and the locus chart of 5 determined allelic enzymes were analyzed to provide a theoretical basis for breeding and resource utilization of Trifolium repens. The results showed that there was great genetic adversity among different germplasm resources. The results from the genetic similarity and clustering analysis among different germplasm resources indicated that 16 Trifolium repens germplasm resources could be divided into three groups based on 0. 71 of similarity coefficient. The germplasm resources from the close geographical regions were clustered in the same group firstly, which indicated that there had the close blood relationship.
出处
《贵州农业科学》
CAS
北大核心
2010年第3期22-24,共3页
Guizhou Agricultural Sciences
