摘要
目的：为了探讨神经丝蛋白在成年型脊肌萎缩症患者活检取材的腓肠神经中的免疫组化特性是否能够提供可信赖和有用的病理诊断信息，以期发现该病在腓肠神经中相应的病理改变特征。方法：对临床与电生理诊断的４例进行性脊肌萎缩症患者行腓肠神经活检，标本经４％多聚甲醛固定，石蜡包埋，切片经ＨＥ，坚牢蓝，银浸染和神经丝蛋白单克隆抗体的ＡＢＣ法免疫组化染色，镜下观察。结果：常规染色与神经特染的镜下主要特点为腓肠神经的轴索变性而髓鞘和许旺氏细胞相对保持完整，免疫组化结果显示轴索中神经丝蛋白明显减少，残留的神经丝呈细丝状或断裂状。结论：成年型进行性脊肌萎缩症的病理特点不仅只表现为脊髓前角神经元的缺失等下运动神经元受损的表现，而且可以累及周围感觉神经，进行性脊肌萎缩症患者的腓肠神经活检病理检查也有相应的特征性改变，即腓肠神经的轴索变性和神经丝蛋白的明显减少。
Objective:In order to test whether immunohistochemical characterization of neurofilament proteins in biopsy of sural nerves gives reliables and useful diagnostic information using neurofilament monoclonal antibodies. Methods: We studied 4 biopy specimens of sural nerves from patients with adult spinal amyotrophy. The clinical features and electroneurophysiologic findings in 4 patients with adult spinal amyorophy were done in all these patients, specimens were fixed in 4% paraformaldehyde-0.1M PBS, embeded in wax and analyzed by HE, Luxol fast blue, Gless-Marsland silver impregnation and immunohistochemical staining of NF monoclonal antibodies (ABC method).Results: In the sural nerve biopsy, the most striking abnormality was the presence of axonal degeneration (axons become fine and breakdown),but the myelin sheath and schwann cells were norman. Conclusion: The results indicated that the pathological findings of adult spinal amyotrophy was not only characterized by lower motor neuron involvement with of anterior horn cells but also with the axonal degeneration and NF decrease in axons of the sural nerves.
出处
《内蒙古医学院学报》
1998年第4期237-239,共3页
Acta Academiae Medicinae Neimongol
关键词
神经丝
免疫组化染色
腓肠神经
肌萎缩
活检
neurofilament
immunoassay
sural nerve
biopsy
muscular atrophy
cytoplasmic filaments
