摘要
目的：了解余姚市感染性腹泻中副溶血性弧菌的感染状况及其研究。方法：采集感染性腹泻病人的大便或肛拭标本,依据GB/T4789.7进行增菌、分离、生化鉴定、血清分型,最后进行药敏试验和PCR毒力基因检测。结果：2007-2008年共检验标本513份,检出副溶血性弧菌43株,检出率为8.38%。药敏试验：43株副溶血性弧菌对阿莫西林棒酸、青霉素、氨苄西林、环丙沙星、头孢唑林、头孢哌酮、庆大霉素7种抗生素的耐药率分别为58.1%、55.8%、、44.2%、7.0%、4.7%、4.7%、4.7%,对另外13种抗生素均敏感。血清分型：43株副溶血性弧菌分布于O1、O2、O3、O4、O6、O11共6个O抗原群；其中以03群为主，占67．4％。毒力基因：有40株副溶血性弧菌检出含有tdh基因，检出率高达93．02％，均未检出trh基因。结论：检出的副溶血性弧菌以03群为主，占67．4％；对阿莫西林棒酸、青霉素耐药性最强，分别为58．1％、55．8％；93．02％的菌珠含有tdh基因，所有菌株均不含trh基因。
Objective：To understand the infective diarrhea in the vice hemolytic vibrio infection status in Yuyao.Methods：Collection of patients with infectious diarrhea or rectal examination of stool specimens.Then enrichment,isolation,biochemical identification,Serotyping,drug susceptibility testing and PCR detection of virulence genes,according to GB/T4789.7.Results：2007-2008 a total of 513 specimens were tested,and the detection of Vibrio parahaemolyticus in 43,with a detection rate of 8.38%.Susceptibility test： 43 strains of Vibrio parahaemolyticus of amoxicillin clavulanic acid,penicillin,ampicillin,ciprofloxacin,cefazolin,cefoperazone,seven kinds of antibiotics,gentamicin resistance rates were 58.1%,55.8%,44.2%,7.0%,4.7%,4.7%,4.7%,on the other 13 kinds of antibiotics are sensitive.Serotyping： 43 Distribution of Vibrio parahaemolyticus in the O1,O2,O3,O4,O6,O11 a total of six O antigen group;of which 03 accounted for 67.4% of the main group.Virulence genes： There were 40 Vibrio parahaemolyticus tdh gene detection with a detection rate of 93.02%,trh gene were not detected.Conclusion：Vibrio parahaemolyticus detection in the 03 groups mainly,accounting for 67.4%;of amoxicillin clavulanic acid,penicillin resistance in the most,namely 58.1%,55.8%;93.02% of bacteria containing beads tdh gene,All strains were non-trh gene.
出处
《中国卫生检验杂志》
CAS
2010年第2期376-378,共3页
Chinese Journal of Health Laboratory Technology
