摘要
目的：建立高效液相色谱法测定面粉及其制品中偶氮甲酰胺的含量。方法：采用Hypersil-C18色谱柱（250 mm×4.6 mm,5μm）,以100%纯水为流动相,流速为1.0 ml/min,检测波长275 nm,进样量20μl。结果：偶氮甲酰胺峰面积对浓度的线性范围0.01-1.0 mg/ml,相关系数r=0.9999,平均回收率（n=6）均大于96.6%,RSD均小于2.0%。结论：该方法简便、准确、重现性好,可用于面粉及其制品中偶氮甲酰胺的测定。
Objective：To establish a method for determining the content of azodicarbonamide in flour and its preparation by RP-HPLC.Methods：Chromatagraphy was conducted on a Hypersil-C18 column（250 mm×4.6 mm,5 μm） at 30℃ and 275 nm.The mobile phase was 100% water at a flow rate of 1.0 ml/min.Results：The calibration curve was linear in the range of 0.01～1.0 mg/ml（r=0.9999）.The average recovery was 96.6% and RSD 2.0%（n=6）.Conclusion：The method is simple and accurate.It can provide a method for quality assessment of ADA in flour and its preparation.
出处
《中国卫生检验杂志》
CAS
2010年第2期274-275,共2页
Chinese Journal of Health Laboratory Technology
